Inferring cell communication using single-cell calcium spatiotemporal dynamics

Abstract

Summary: There are a limited number of experimental tools for non-destructively discovering cell communication events in vitro and in vivo. Here, using tissue-specific genetically encoded calcium indicator (GECI) mice, we describe a protocol for preprocessing GECI fluorescence time-series measured by live cell imaging or intravital microscopy, detecting peaks of single-cell calcium fluorescence transients, and inferring putative cell communication events from peak synchrony.For complete details on the use and execution of this protocol, please refer to Taghdiri et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Bioinformatics
• Cell biology
• Microscopy
• Model organisms
• Molecular biology
• Signal transduction