Circadian miR-218-5p targets gene CA2 to regulate uterine carbonic anhydrase activity during egg shell calcification

Xiaxia Du; Zhifu Cui; Zifan Ning; Xun Deng; Felix Kwame Amevor; Gang Shu; Xiaoqi Wang; Zhichao Zhang; Yaofu Tian; Qing Zhu; Yan Wang; Diyan Li; Yao Zhang; Xiaoling Zhao

Poultry Science (Nov 2022)

Circadian miR-218-5p targets gene CA2 to regulate uterine carbonic anhydrase activity during egg shell calcification

Xiaxia Du,
Zhifu Cui,
Zifan Ning,
Xun Deng,
Felix Kwame Amevor,
Gang Shu,
Xiaoqi Wang,
Zhichao Zhang,
Yaofu Tian,
Qing Zhu,
Yan Wang,
Diyan Li,
Yao Zhang,
Xiaoling Zhao

Affiliations

Xiaxia Du: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Zhifu Cui: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Zifan Ning: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Xun Deng: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Felix Kwame Amevor: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Gang Shu: Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China
Xiaoqi Wang: Agriculture and Animal Husbandry Comprehensive Service Center, Tibet Autonomous Region, P. R. China
Zhichao Zhang: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Yaofu Tian: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Qing Zhu: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Yan Wang: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Diyan Li: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Yao Zhang: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China
Xiaoling Zhao: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, P. R. China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agricultural and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, P. R., Chengdu, China; Corresponding author:

Journal volume & issue: Vol. 101, no. 11
p. 102158

Abstract

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ABSTRACT: MicroRNAs (miRNAs) are involved in regulating the circadian clock. In our previous work, miR-218-5p was found to be a circadian miRNA in the chicken uterus, but its role in the eggshell formation process was not clear. In the present study, we found that the expression levels of miR-218-5p and two 2 predicted target genes carbonic anhydrase 2 (CA2) and neuronal PAS domain protein 2 (NPAS2) were oscillated in the chicken uterus. The results of dual-luciferase reporter gene assays in the present study demonstrated that miR-218-5p directly targeted the 3’ untranslated regions of CA2 and NPAS2. miR-218-5p showed an opposite expression profile to CA2 within a 24 h cycle in the chicken uterus. Moreover, over-expression of miR-218-5p reduced the mRNA and protein expression of CA2, while miR-218-5p knockdown increased CA2 mRNA and protein expression. Overexpression of CA2 also significantly increased the activity of carbonic anhydrase Ⅱ (P < 0.05), whereas knockdown of CA2 decreased the activity of carbonic anhydrase Ⅱ. miR-218-5p influenced carbonic anhydrase activity via regulating the expression of CA2. These results demonstrated that clock-controlled miR-218-5p regulates carbonic anhydrase activity in the chicken uterus by targeting CA2 during eggshell formation.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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