Phosphodiesterases Inhibition by Bacilli Calmette-Guérin Contributes to Decrease Asthma in Allergic Rats

Yajuan Wang; Yali Jiang; Huifang Tang; Xuefeng Wang; Chunzhen Zhao; Jiqiang Chen

Iranian Journal of Allergy, Asthma and Immunology (Sep 2011)

Phosphodiesterases Inhibition by Bacilli Calmette-Guérin Contributes to Decrease Asthma in Allergic Rats

Yajuan Wang,
Yali Jiang,
Huifang Tang,
Xuefeng Wang,
Chunzhen Zhao,
Jiqiang Chen

Affiliations

Yajuan Wang: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310058, China AND The Center of Function Experiment, Anhui College of Traditional Chinese Medicine, Hefei, 230038, China
Yali Jiang: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310058, China
Huifang Tang: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310058, China
Xuefeng Wang: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310058, China
Chunzhen Zhao: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310058, China
Jiqiang Chen: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310058, China

Journal volume & issue: Vol. 10, no. 3

Abstract

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Phosphodiesterases (PDE) hydrolyse intracellular cAMP and cGMP to inactive 5’ monophosphates. Decreased level of cAMP is involved in the pathogenesis of asthma. We and others have shown that phosphodiesterases were upregulated in the lung of allergic rats, and Bacilli Calmette-Guérin (BCG) induced the production of cAMP in vitro. However, it is unclear how BCG’s effect asthma and whether it is related to PDEs. In this study, BCG was intraperitoneally injected into male Sprague-Dawley rats sensitized and later the rats were challenged with ovabumin/pertusis. The inflammation in lungs was measured. Airway hyperresponsiveness was determined using MedLab software after intravenous methacholine challenge. Furthermore, cAMP level and adenylate cyclase activity in lungs were analyzed by ELISA, phosphodiesterases activities were analyzed by HPLC, while PDEs mRNA levels in lungs was analyzed by reverse transcription-polymerase chain reaction. Administration of BCG significantly attenuated allergen-induced lung inflammatory response and hyper responsiveness as compared with vehicle treatment. Furthermore, the levels of cAMP in lungs were significantly increased in BCG-treated allergic rats. Interestingly, administration of BCG decreased the activity of cAMP-PDE, but not adenylyl cyclase (AC), activity in lungs of animals. Furthermore, pretreatment with BCG significantly decreased the mRNA levels of PDE4A, 4C, 5 and 8, which were induced in lungs of allergic rats. BCG administration attenuated airway inflammatory response and bronchial hyper responsiveness in rats, which are the most important symptoms in asthma. The decreased PDEs mRNA and inhibited cAMP-PDE activities by BCG contribute, at least in part, prevention of allergen-induced airway inflammation and asthma in rats.

Published in Iranian Journal of Allergy, Asthma and Immunology

ISSN: 1735-1502 (Print); 1735-5249 (Online)
Publisher: Tehran University of Medical Sciences
Country of publisher: Iran, Islamic Republic of
LCC subjects: Medicine
Website: https://ijaai.tums.ac.ir

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