Frontiers in Cellular and Infection Microbiology (Aug 2021)

Combined Signature of the Urinary Microbiome and Metabolome in Patients With Interstitial Cystitis

  • Hewei Xu,
  • Hewei Xu,
  • Nebiyu Elias Tamrat,
  • Nebiyu Elias Tamrat,
  • Jie Gao,
  • Jie Gao,
  • Jie Xu,
  • Jie Xu,
  • Yiduo Zhou,
  • Yiduo Zhou,
  • Sicong Zhang,
  • Sicong Zhang,
  • Zhengsen Chen,
  • Zhengsen Chen,
  • Yunpeng Shao,
  • Yunpeng Shao,
  • Liucheng Ding,
  • Liucheng Ding,
  • Baixin Shen,
  • Baixin Shen,
  • Zhongqing Wei,
  • Zhongqing Wei

DOI
https://doi.org/10.3389/fcimb.2021.711746
Journal volume & issue
Vol. 11

Abstract

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Interstitial cystitis (IC) is a clinical syndrome characterized by frequency, urgency, and bladder pain or pelvic pain; however, the underlying pathophysiological mechanisms and diagnostic markers are unknown. In this study, microbiome and metabolome analysis were used to explain the urine signatures of IC patients. Urine samples from 20 IC patients and 22 control groups were analyzed by using 16S rRNA sequence and liquid chromatography coupled with mass spectrometry. Four opportunistic pathogen genera, including Serratia, Brevibacterium, Porphyromonas, and Citrobacter, were significantly upregulated in IC group. The altered metabolite signatures of the metabolome may be related to sphingosine metabolism, amino acid metabolism, and fatty acid biosynthesis. Meanwhile, the associations were observed between different metabolites and microbiomes of IC. The present study suggests that the combined signatures of IC in urine microbiome and metabolome may become its prospective diagnostic markers.

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