Ectopic expression of an apple ABCG transporter gene MdABCG25 increases plant cuticle wax accumulation and abiotic stress tolerance

Min-Min Zhou; Zi-Han Yu; Huai-Na Gao; Meng-Ru Li; Ya-Ting Wu; Hai-Yang Li; Tao Wang; Yan-Hui Lv; Han Jiang; Yuan-Yuan Li

doi:10.48130/FruRes-2023-0043

Fruit Research (Jan 2023)

Ectopic expression of an apple ABCG transporter gene MdABCG25 increases plant cuticle wax accumulation and abiotic stress tolerance

Min-Min Zhou,
Zi-Han Yu,
Huai-Na Gao,
Meng-Ru Li,
Ya-Ting Wu,
Hai-Yang Li,
Tao Wang,
Yan-Hui Lv,
Han Jiang,
Yuan-Yuan Li

Affiliations

Min-Min Zhou: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Zi-Han Yu: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Huai-Na Gao: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Meng-Ru Li: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Ya-Ting Wu: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Hai-Yang Li: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Tao Wang: Tai'an Institute for Food and Drug Control, Tai-An 271000, Shandong, China
Yan-Hui Lv: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Han Jiang: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
Yuan-Yuan Li: National Key Laboratory of Wheat Improvement, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, National Research, Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China

DOI: https://doi.org/10.48130/FruRes-2023-0043
Journal volume & issue: Vol. 3, no. 1
pp. 1 – 13

Abstract

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A number of enzymes and transcription factors involved in cuticular wax biosynthesis have been identified in numerous plant species. The pathway of wax biosynthesis is relatively definite. The molecular basis of cuticular wax deposition is still unclear, especially the transport mechanism of cuticular wax from endoplasmic reticulum (ER) to the cell wall. It has been found that the ABCG transporter family is involved in the wax export process in some model plants. However, whether ABCG transporters participate in wax transport in apple is unknown. In this study, MdABCG25, encoding an ABCG transporter, was isolated from apple (Malus domestica Brokh.). MdABCG25, containing an AAA domain and an ABC2_membrane domain, is highly expressed in apple pericarp and induced by drought, salt and ABA conditions. The heterologous expression of MdABCG25 in Arabidopsis leads to an increasing cuticular wax accumulation of stems and leaves. The epidermal permeability, water loss rate and chlorophyll extraction rate of MdABCG25 transgenic Arabidopsis were decreased. The expression of MdABCG25 improved the drought and salt tolerance of apple calli and Arabidopsis.

Published in Fruit Research

ISSN: 2769-4615 (Online)
Publisher: Maximum Academic Press
Country of publisher: China
LCC subjects: Agriculture: Plant culture
Website: https://www.maxapress.com/frures

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