A protocol for full-rotation soft X-ray tomography of single cells

Jian-Hua Chen; Bieke Vanslembrouck; Valentina Loconte; Axel Ekman; Mirko Cortese; Ralf Bartenschlager; Gerry McDermott; Carolyn A. Larabell; Mark A. Le Gros; Venera Weinhardt

STAR Protocols (Mar 2022)

A protocol for full-rotation soft X-ray tomography of single cells

Jian-Hua Chen,
Bieke Vanslembrouck,
Valentina Loconte,
Axel Ekman,
Mirko Cortese,
Ralf Bartenschlager,
Gerry McDermott,
Carolyn A. Larabell,
Mark A. Le Gros,
Venera Weinhardt

Affiliations

Jian-Hua Chen: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Bieke Vanslembrouck: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Valentina Loconte: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Axel Ekman: Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Mirko Cortese: Department of Infectious Diseases, Molecular Virology Heidelberg University, Heidelberg 69120, Germany
Ralf Bartenschlager: Department of Infectious Diseases, Molecular Virology Heidelberg University, Heidelberg 69120, Germany; German Center for Infection Research, Heidelberg Partner Site, Heidelberg 69120, Germany; Division Virus-Associated Carcinogenesis, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany
Gerry McDermott: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Carolyn A. Larabell: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Mark A. Le Gros: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
Venera Weinhardt: Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Centre for Organismal Studies, Heidelberg University, Heidelberg 69120, Germany; Corresponding author

Journal volume & issue: Vol. 3, no. 1
p. 101176

Abstract

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Summary: The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes.For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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