Latent atherogenic dyslipoproteinemia diagnostics by genetic analysis of monozygotic twins

Abstract

Aim. To analyze intra-pair correlation of lipid-transport system parameters in fasting state and after food lipid load (FLL) in monozygotic twins (MT) with different body mass (BM), for identifying genetic and environmental factor role in atherogenic dyslipidemia development. Material and methods. Nine pairs (n=18) of male and female MT aged 37-65 years were divided into two groups, by abdominal obesity (AO) presence (n=9) or absence (n=6). Three persons with gluteo-femoral obesity were excluded from the analysis. In all patients, standard FLL test was performed, with measurement of TCH, TG, HDL-CH, LDL-CH, apoAI, apoB, and their ratio, HDL components (CH, phospholipids, CH esters), and CH-acceptor HDL potential 3 and 6 hours after FLL test. Results. In MT with or without AO, lipid and apoprotein LP parameters, as well as CH-acceptor HDL potential, were similar to those in previously examined large groups of patients with various obesity types. In MT with AO, small particles were more prevalent in sub-fraction HDL specter than in MT with normal BM. In MT, atherogenic LP parameters correlated with one another, including TCH, apoB, АТ (apoB/AI), LDL particle size in fasting state and after FLL. No correlation for fasting antiatherogenic HDL components was observed in MT. After FLL, HDL level and content, according to cell culture CH-acceptor HDL potential, began to correlate. Conclusion. Lipid and apoprotein atherogenic LDL parameters in fasting state and after FLL were determined mostly by genetic factors. Levels and content of antiatherogenic HDL are mostly determined by environmental factors; their post-FLL dynamics is genetically determined.

Keywords

• lipids
• twin analysis
• obesity
• lipid load