Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice

Abstract

Objective To study the effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice. Methods Twelve 12-week-old male APP/PS1 double transgenic mice were randomly divided into the control group (C group) and the 50 Hz magnetic field exposure group (MF group) (n=6). The mice in the MF group were exposed to 1.0 mT 50 Hz magnetic field 2 h/d for 3 weeks (21 d). The mice in the sham exposure group had the same conditions as those in the MF group, except for no magnetic field exposure. After exposure, the cerebral cortex tissues of mice were taken according to the standard method for high-resolution untargeted metabonomics tests, including sample metabolite extraction, sample LC-MS/MS mass spectrometry analysis, metabolite qualitative and quantitative analysis and data analysis. Results The growth of APP/PS1 double transgenic mice were not affected after 50 Hz magnetic field exposure. The high-resolution untargeted metabonomics showed 1 068 metabolites were identified. Based on fold change analysis (FC>1.5 or FC 1, P < 0.05), 30 identified significant changed metabolites were screened in cerebral cortex of mice after 50 Hz magnetic field exposure. And the top 3 significant changed metabolites in VIP value were 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (VIP=19.76, FC=0.52), dehydroascorbic acid (VIP=12.71, FC=0.72) and inosine (VIP=10.64, FC=1.05). The analyses of hierarchical clustering, correlation and Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that the 30 VIP significant changed metabolites may be involved in 16 KEGG pathways. L-aspartic acid, L-alanine and acetylcholine may be involved in 13, 7 and 3 pathways of the 16 KEGG pathways respectively. Conclusion The amino acid metabolism, ATP-binding cassette transporters, CoA and aminoacyl-tRNA biosynthesis, regulation of actin cytoskeleton and neuroactive ligand-receptor interaction may be impacted in cerebral cortex of APP/PS1 transgenic mice by 50 Hz magnetic field exposure.

Keywords

• 50 hz magnetic field
• app/ps1 transgenic mice
• cerebral cortex
• untargeted metabolomics