Pooled library screening with multiplexed Cpf1 library

Jintan Liu; Sanjana Srinivasan; Chieh-Yuan Li; I-Lin Ho; Johnathon Rose; MennatAllah Shaheen; Gang Wang; Wantong Yao; Angela Deem; Chris Bristow; Traver Hart; Giulio Draetta

doi:10.1038/s41467-019-10963-x

Nature Communications (Jul 2019)

Pooled library screening with multiplexed Cpf1 library

Jintan Liu,
Sanjana Srinivasan,
Chieh-Yuan Li,
I-Lin Ho,
Johnathon Rose,
MennatAllah Shaheen,
Gang Wang,
Wantong Yao,
Angela Deem,
Chris Bristow,
Traver Hart,
Giulio Draetta

Affiliations

Jintan Liu: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
Sanjana Srinivasan: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
Chieh-Yuan Li: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
I-Lin Ho: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
Johnathon Rose: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
MennatAllah Shaheen: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
Gang Wang: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Wantong Yao: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center
Angela Deem: Institute for Applied Cancer Science, The University of Texas MD Anderson Cancer Center
Chris Bristow: Institute for Applied Cancer Science, The University of Texas MD Anderson Cancer Center
Traver Hart: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Giulio Draetta: Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center

DOI: https://doi.org/10.1038/s41467-019-10963-x
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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