Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis

Abstract

In order to conserve sugarcane germplasm, produce microbial disease-free material, detect resistance to diseases and pests, etc., in vitro propagation of sugarcane has been established in many commercial varieties. In this sense, the aim of this work was to analyze the efficiency of 2,4-dichlorophenoxyacetic acid (2,4D) and 3,6-dichloro -2- methoxybenzoic acid (Dicamba) to induce somatic embryogenesis and regeneration of plantlets from sugarcane varieties C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475. For induction of embryogenic callus, leaf discs in tillering stage of 1 cm diameter and 2 mm thick, were inoculated on Murashige-Skoog, 1962 medium (MS), supplemented with 50 ml.l-1 coconut water, 30 g.l-1sucrose and two different treatments: 3 mg.l-1 2,4D or 6.63 mg.l-1 Dicamba, both of them in total darkness at 25 °C, during 1 month. For plant regeneration, embryogenic calli were transferred to ½ MS salts supplemented with coconut water 200 ml.l-1 and sucrose 60 g.l-1 and incubated under continuous light, 25 °C, for 2 months. The highest percent of embryogenic callus induction was obtained in media supplemented with Dicamba, an average of 70.83 % of embryogenic callus by variety, while in media with 2,4-D, 62.08 % of embryogenic callus was obtained by variety. An average of 89,00 % of plantlets was obtained from calli induced on media with Dicamba and an average of 66.12% of plantlets was obtained from calli induced on media supplemented with 2,4D. Using Dicamba it was possible to establish an efficient somatic embryogenesis protocol for these sugarcane varieties.

Keywords

• Cultivo in vitro
• auxinas
• vitroplántulas
• Sacharum spp.
• embriogénesis somática
• auxins
• vitroplantlets
• Sacharum spp.
• somatic embryos