علوم بهداشتی ایران (Mar 2016)
Evaluation of Efficacy of the Current Disinfectants on Gram-negative Bacteria Isolated from Hospital in Yazd in 2014
Abstract
as Pseudomonas, Acinetobacter, and Staphylococcus that appropriate disinfection can reduce these pathogens. The aim of this study was to evaluate the effect of different disinfectants on Gram-negative bacteria isolated from the surface of accidents and burn hospital in Yazd. Materials and Methods: In this study, 240 samples were randomly collected from different parts of accidents and burn hospital before and after disinfection. The samples were cultured on blood agar and Eusion-Metilen-Blue agar media in the Microbiology Laboratory of Medicine School of Shahid Sadoughi University in Yazd and Colony counting were determined. Identification was done by biochemical tests after incubation at 37° C for 48 hours. The studied disinfectants were Deconex 50AF, Descoscid, Epimax SC, and Silvosept. At last, data were analyzed with using paired t-test. Results: The Gram-negative bacteria were isolated from burn unit before disinfection included Pseudomonas aeruginosa, Escherichia coli, Proteus spp., Klebsiella spp., Acinetobacter spp., and Enterobacter spp. According to the results, all disinfectants reduced the pollution before and after disinfection; nevertheless, this reduction at the time of using Epimax SC and Silvosept only showed a significant difference for P. aeruginosa (P = 0.001 and 0.003) and for E. coli (P = 0.020 and 0.005), respectively. Conclusion: All disinfectants were effective on Gram-negative bacteria isolated from surfaces and had shown a significant difference only between P. aeruginosa and E. coli number before and after disinfection by Epimax SC and Silvosept. The most effective disinfectant on P. aeruginosa and E. coli was Epimax SC and Silvosept, respectively.
