Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media

Sylvain Perriot; Mathieu Canales; Amandine Mathias; Renaud Du Pasquier

STAR Protocols (Dec 2021)

Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media

Sylvain Perriot,
Mathieu Canales,
Amandine Mathias,
Renaud Du Pasquier

Affiliations

Sylvain Perriot: Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland; Corresponding author
Mathieu Canales: Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland
Amandine Mathias: Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland
Renaud Du Pasquier: Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland; Service of Neurology, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100902

Abstract

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Summary: This protocol describes how to obtain human astrocytes from human-induced pluripotent stem cells (hiPSCs) in chemically defined media, without the use of fetal bovine serum (FBS). FBS eases the differentiation of astrocytes but also deeply alters their phenotype, as compared with their in vivo counterparts. Our protocol generates hiPSC-derived astrocytes displaying a phenotype and functions similar to human primary astrocytes, including adequate response to inflammation, neurotransmitter uptake, and trophic support to neurons.For complete details on the use and execution of this protocol, please refer to Perriot et al. (2018).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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