Antibody Profiling of Microbial Antigens in the Blood of COVID-19 mRNA Vaccine Recipients Using Microbial Protein Microarrays

Hiroaki Saito; Hiroki Yoshimura; Makoto Yoshida; Yuta Tani; Moe Kawashima; Taiga Uchiyama; Tianchen Zhao; Chika Yamamoto; Yurie Kobashi; Toyoaki Sawano; Seiya Imoto; Hyeongki Park; Naotoshi Nakamura; Shingo Iwami; Yudai Kaneko; Aya Nakayama; Tatsuhiko Kodama; Masatoshi Wakui; Takeshi Kawamura; Masaharu Tsubokura

doi:10.3390/vaccines11111694

Vaccines (Nov 2023)

Antibody Profiling of Microbial Antigens in the Blood of COVID-19 mRNA Vaccine Recipients Using Microbial Protein Microarrays

Hiroaki Saito,
Hiroki Yoshimura,
Makoto Yoshida,
Yuta Tani,
Moe Kawashima,
Taiga Uchiyama,
Tianchen Zhao,
Chika Yamamoto,
Yurie Kobashi,
Toyoaki Sawano,
Seiya Imoto,
Hyeongki Park,
Naotoshi Nakamura,
Shingo Iwami,
Yudai Kaneko,
Aya Nakayama,
Tatsuhiko Kodama,
Masatoshi Wakui,
Takeshi Kawamura,
Masaharu Tsubokura

Affiliations

Hiroaki Saito: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Hiroki Yoshimura: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Makoto Yoshida: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Yuta Tani: Medical Governance Research Institute, Minato-ku, Tokyo 108-0074, Japan
Moe Kawashima: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Taiga Uchiyama: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Tianchen Zhao: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Chika Yamamoto: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Yurie Kobashi: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Toyoaki Sawano: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan
Seiya Imoto: Division of Health Medical Intelligence, Human Genome Center, Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
Hyeongki Park: Interdisciplinary Biology Laboratory (iBLab), Division of Natural Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
Naotoshi Nakamura: Interdisciplinary Biology Laboratory (iBLab), Division of Natural Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
Shingo Iwami: Interdisciplinary Biology Laboratory (iBLab), Division of Natural Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
Yudai Kaneko: Medical & Biological Laboratories Co., Ltd., Minato-ku, Tokyo 105-0012, Japan
Aya Nakayama: Isotope Science Centre, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
Tatsuhiko Kodama: Laboratory for Systems Biology and Medicine, Research Centre for Advanced Science and Technology (RCAST), The University of Tokyo, Meguro-ku, Tokyo 153-8904, Japan
Masatoshi Wakui: Department of Laboratory Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan
Takeshi Kawamura: Laboratory for Systems Biology and Medicine, Research Centre for Advanced Science and Technology (RCAST), The University of Tokyo, Meguro-ku, Tokyo 153-8904, Japan
Masaharu Tsubokura: Department of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960-1247, Japan

DOI: https://doi.org/10.3390/vaccines11111694
Journal volume & issue: Vol. 11, no. 11
p. 1694

Abstract

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Although studies have demonstrated that infections with various viruses, bacteria, and parasites can modulate the immune system, no study has investigated changes in antibodies against microbial antigens after the COVID-19 mRNA vaccination. IgG antibodies against microbial antigens in the blood of vaccinees were comprehensively analyzed using microbial protein microarrays that carried approximately 5000 microbe-derived proteins. Changes in antibodies against microbial antigens were scrutinized in healthy participants enrolled in the Fukushima Vaccination Community Survey conducted in Fukushima Prefecture, Japan, after their second and third COVID-19 mRNA vaccinations. Antibody profiling of six groups stratified by antibody titer and the remaining neutralizing antibodies was also performed to study the dynamics of neutralizing antibodies against SARS-CoV-2 and the changes in antibodies against microbial antigens. The results showed that changes in antibodies against microbial antigens other than SARS-CoV-2 antigens were extremely limited after COVID-19 vaccination. In addition, antibodies against a staphylococcal complement inhibitor have been identified as microbial antigens that are associated with increased levels of neutralizing antibodies against SARS-CoV-2. These antibodies may be a predictor of the maintenance of neutralizing antibodies following the administration of a COVID-19 mRNA vaccine.

Published in Vaccines

ISSN: 2076-393X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/vaccines

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