Scientific Reports (Jul 2024)

Flavonoids and their relationship with the physiological quality of seeds from different soybean genotypes

  • Izabela Cristina de Oliveira,
  • Dthenifer Cordeiro Santana,
  • João Lucas Gouveia de Oliveira,
  • Elber Vinícius Martins Silva,
  • Ana Carina da Silva Candido Seron,
  • Matildes Blanco,
  • Larissa Pereira Ribeiro Teodoro,
  • Carlos Antônio da Silva Júnior,
  • Fabio Henrique Rojo Baio,
  • Charline Zaratin Alves,
  • Paulo Eduardo Teodoro

DOI
https://doi.org/10.1038/s41598-024-68117-z
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 8

Abstract

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Abstract Flavonoids are compounds that result from the secondary metabolism of plants and play a crucial role in plant development and mitigating biotic and abiotic stresses. The highest levels of flavonoids are found in legumes such as soybean. Breeding programs aim to increase desirable traits, such as higher flavonoid contents and vigorous seeds. Soybeans are one of the richest sources of protein in the plant kingdom and the main source of flavonoid derivatives for human health. In view of this, the hypothesis of this study is based on the possibility that the concentration of isoflavones in soybean seeds contributes to the physiological quality of the seeds. The aim of this study was to analyze the content of flavonoids in soybean genotypes and their influence on the physiological quality of the seeds. Seeds from thirty-two soybean genotypes were obtained by carrying out a field experiment during the 2021/22 crop season. The experimental design was randomized blocks with four replications and thirty-two F3 soybean populations. The seeds obtained were subjected to germination, first germination counting, electrical conductivity and tetrazolium vigor and viability tests. After drying and milling the material from each genotype, liquid chromatography analysis was carried out to obtain flavonoids, performed at UPLC level. Data were submitted to analysis of variance and, when significant, the means were compared using the Scott-Knott test at 5% probability. The results found here show the occurrence of genotypes with higher amounts of flavonoids when compared to their peers. The flavonoid FLVD_G2 had the highest concentration and differed from the others. Thus, we can assume that the type and concentration of flavonoids does not influence the physiological quality of seeds from different soybean genotypes, but it does indirectly contribute to viability and vigor, since the genotypes with the highest FLVD_G2 levels had better FGC values. The findings indicate that there is a difference between the content of flavonoids in soybean genotypes, with a higher content of genistein. The content of flavonoids does not influence the physiological quality of seeds, but contributes to increasing viability and vigor.

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