Molecular Cancer (May 2023)

Deciphering and advancing CAR T-cell therapy with single-cell sequencing technologies

  • Shengkang Huang,
  • Xinyu Wang,
  • Yu Wang,
  • Yajing Wang,
  • Chenglong Fang,
  • Yazhuo Wang,
  • Sifei Chen,
  • Runkai Chen,
  • Tao Lei,
  • Yuchen Zhang,
  • Xinjie Xu,
  • Yuhua Li

DOI
https://doi.org/10.1186/s12943-023-01783-1
Journal volume & issue
Vol. 22, no. 1
pp. 1 – 25

Abstract

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Abstract Chimeric antigen receptor (CAR) T-cell therapy has made remarkable progress in cancer immunotherapy, but several challenges with unclear mechanisms hinder its wide clinical application. Single-cell sequencing technologies, with the powerful unbiased analysis of cellular heterogeneity and molecular patterns at unprecedented resolution, have greatly advanced our understanding of immunology and oncology. In this review, we summarize the recent applications of single-cell sequencing technologies in CAR T-cell therapy, including the biological characteristics, the latest mechanisms of clinical response and adverse events, promising strategies that contribute to the development of CAR T-cell therapy and CAR target selection. Generally, we propose a multi-omics research mode to guide potential future research on CAR T-cell therapy.

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