Survival of bovine blastocysts produced in vitro and vitrified in vitri-tip and vitri-top devices.

Abstract

Vitrification is a new technique that has many potential advantages in mammalian oocytes and embryos. In the present work, the objective was to compare of post thawing survival rate by of blastocysts vitrified in vitro in two devices. Standardized procedure at the Laboratory of Reproductive Biotechnology, UNA La Molina was used in the experimental part, to produce in vitro bovine embryos from oocytes recovered from slaughterhouse ovaries. The viable oocytes were incubated at 38.5 ° C and 5% CO2 in 10 oocytes/group microdroplet (70 µl) covered with mineral oil, for 22 to 24 hours in maturation medium, 18 hours in fertilization medium and 7 days in culture medium. A total of 100 expanded blastocysts produced bovine day 7 of culture were used. Blastocysts were vitrified in Vitritip (n = 50) and Vitri-top (n= 50) devices, using standard media (Vitrogen®, Brazil) on vitrification, thawing and culture in vitro. Once thawed blastocysts from both groups were cultured in vitro for 3 hours at 38.5 ° C, 5% CO2 and 90% Hd. The comparison of both groups was performed using the statistical test Chi-square and using a significance level of 5%. No statistically significant differences (P>0.05) in the re-expansion rates in vitro post thawing between both devices during vitrification were observed post thawing survival rate, 52% (24/46) Vitri-tip and 48% (21/44) Vitri-top devices. Devices using vitrification closed in bovine embryos, can achieve low loss rate in low volume management, although the embryonic survival was not very impressive.

Keywords

• Bovine
• blastocyst
• vitrification
• desvices