Nature Communications (Jan 2023)
DNA binding and RAD51 engagement by the BRCA2 C-terminus orchestrate DNA repair and replication fork preservation
- Youngho Kwon,
- Heike Rösner,
- Weixing Zhao,
- Platon Selemenakis,
- Zhuoling He,
- Ajinkya S. Kawale,
- Jeffrey N. Katz,
- Cody M. Rogers,
- Francisco E. Neal,
- Aida Badamchi Shabestari,
- Valdemaras Petrosius,
- Akhilesh K. Singh,
- Marina Z. Joel,
- Lucy Lu,
- Stephen P. Holloway,
- Sandeep Burma,
- Bipasha Mukherjee,
- Robert Hromas,
- Alexander Mazin,
- Claudia Wiese,
- Claus S. Sørensen,
- Patrick Sung
Affiliations
- Youngho Kwon
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Heike Rösner
- Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen
- Weixing Zhao
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Platon Selemenakis
- Department of Environmental and Radiological Health Sciences, Colorado State University
- Zhuoling He
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Ajinkya S. Kawale
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Jeffrey N. Katz
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Cody M. Rogers
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Francisco E. Neal
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Aida Badamchi Shabestari
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Valdemaras Petrosius
- Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen
- Akhilesh K. Singh
- Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine
- Marina Z. Joel
- Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine
- Lucy Lu
- Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine
- Stephen P. Holloway
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Sandeep Burma
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Bipasha Mukherjee
- Department of Neurosurgery, University of Texas Health Science Center at San Antonio
- Robert Hromas
- Department of Medicine, University of Texas Health at San Antonio
- Alexander Mazin
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- Claudia Wiese
- Department of Environmental and Radiological Health Sciences, Colorado State University
- Claus S. Sørensen
- Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen
- Patrick Sung
- Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio
- DOI
- https://doi.org/10.1038/s41467-023-36211-x
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 18
Abstract
Exon 27 of the tumor suppressor BRCA2 encodes a portion of the protein crucial for DNA repair, genome maintenance, and tumor suppression. Here the authors show that this domain binds DNA and the RAD51 recombinase to enhance the assembly of RAD51-DNA complexes.
