Biotechnic of brood stocking, artificial propagation and some physiological indices in farmed Acipenser nudiventris

Abstract

This study was conducted on farmed Acipenser nudiventris at the International Sturgeon Research Institute from the winter of 2007 till summer 2010. A total of 11 farmed A. nudiventris specimens (7 male and 4 female) were stocked in groups of 4 fish in eight fiberglass tanks (4 tons capacity) on the basis of their sex. Males were fed with a diet with no soybean but containing vitamins. Females were fed with a diet containing soybean and vitamins with 38-40% protein, 13-15% fat, 19.5-20Mj/kg energy, respectively. GnRH was used for artificial propagation of Acipenser nudiventris brood stocks twice for females (dose 10μg/kg with 80:20 ration) and during one occasion for males with a dose of 20 μg/kg. The results of the study indicated that food composition (soybean and vitamins C and E) played a significant and positive role in the reproduction system for females. No significant effect of treatment was observed in Testosterone levels between mature (Mean ±SD: 60.6±8.07ng/ml) and immature (24.42±4.87ng/ml) males, Likewise, no significant differences were found in17α-Hydroxy Progestrone for female (Mean ±SD: 0.106±0.019ng/ml) and for immature fishes (0.031±0.006ng/ml) significant differences was detected in the concentration of Albomine (1.54±0.05mg/dl for females and 1.35±0.07mg/dl for immature females), but there were no significant differences in other biochemical parameters including Glocose, Cortisol, Chlosterole and Triglisride in males and females. There were no significant differences in total weight and length, WBC, RBC, Hb, Hct, MCH, MCHC and MCV parameters in males and females. This study is a step forward towards the development of artificial breeding and rearing sturgeon fish in the country.

Keywords

• Sexual hormones; Reproductive physiology; Sturgeon fish breeding