TP901-1 Phage Recombinase Facilitates Genome Engineering in Drosophila melanogaster

Abstract

Molecular biology techniques have a large impact on biomedical research and the availability of diverse tools to perform genome manipulations advances the ease of executing complicated genetic research. Here, we introduce in the fruit fly another such tool by harnessing the phage recombinase TP901-1 to perform site-directed recombination that leads to recombinase-mediated cassette exchange (RMCE). The TP901-1 system complements already existing recombination systems and enhances genome engineering in the fruit fly and other organisms.

Keywords

• TP901-1
• site-directed recombinase
• cassette exchange
• CRISPR/Cas9
• genome engineering
• Drosophila melanogaster