Chalcone isomerase in grape vine: gene expression and localization in the developing fruit

W. Wang; H. -L. Wang; S. -B. Wan; J. -H. Zhang; P. Zhang; J. -C. Zhan; W. -D. Huang

doi:10.1007/s10535-011-0216-2

Biologia Plantarum (Sep 2012)

Chalcone isomerase in grape vine: gene expression and localization in the developing fruit

W. Wang,
H. -L. Wang,
S. -B. Wan,
J. -H. Zhang,
P. Zhang,
J. -C. Zhan,
W. -D. Huang

Affiliations

W. Wang: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
H. -L. Wang: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
S. -B. Wan: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
J. -H. Zhang: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
P. Zhang: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
J. -C. Zhan: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
W. -D. Huang: Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China

DOI: https://doi.org/10.1007/s10535-011-0216-2
Journal volume & issue: Vol. 56, no. 3
pp. 545 – 550

Abstract

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Chalcone isomerase (CHI, EC 5.5.1.6) is an entrance enzyme in the flavonoid biosynthesis, which catalyzes the conversion of chalcones to flavanones. In this study, the full-length CHI cDNA from grape vine (Vitis vinifera L.) was cloned, the recombinant protein was purified and the polyclonal antibody was prepared. Using these tools, the expression and tissue localization of CHI in developing grape berry was analyzed by RT-PCR, gel blot hybridization and immunohistochemical techniques. The expression of CHI was dependent on developmental stage, and CHI protein was mainly distributed in vascular bundles throughout all the stages of berry development, which suggested that flavonoids in the berry might have been partially synthesized in situ.

Published in Biologia Plantarum

ISSN: 1573-8264 (Online)
Publisher: Institute of Experimental Botany of the Czech Academy of Sciences
Country of publisher: Czechia
LCC subjects: Science: Biology (General); Science: Botany: Plant ecology
Website: https://bp.ueb.cas.cz/

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