Fungitoxic Potential of Extracts of Four Pinus spp. Bark to Inhibit Fungus Trametes versicolor (L. ex. Fr.) Pilát

Abstract

Antifungal potential of bark crude extracts of Pinus strobus L., Pinus douglasiana Martinez, Pinus caribaea Morelet [var. Hondurensis (scheclauze) W. H. G. Barrett & Golfari] and Pinus leiophylla Schltdl. & Cham, for inhibiting mycelial growth to fungus Trametes versicolor (L. ex Fr.) Pilát was investigated. Pinus bark was extracted through a mixture of acetone-hexane-water 54:44:2 V. Bioassays were performed according to the agar dilution method (petri dish) using concentrations of 0.1, 0.5, and 1.0 mg mL-1 for each of the obtained extracts. All extracts showed some inhibition degree. Crude extracts of P. strobus bark showed the greatest inhibition degree. Antifungal potential of all tested extracts at a concentration of 1.0 mg mL-1 was classified as toxic. Through simple regression models, concentrations were calculated in order to achieve a 50% inhibition (IC50) and the minimal inhibition concentration (MIC). MIC values were 1.58, 1.72, 1.24, and 1.94 mg mL-1 for bark extracts of P. strobus, P. douglasiana, P. caribaea, and P. leiophylla, respectively.

Keywords

• Antifungal potential
• Bark extracts
• Pine bark
• Trametes versicolor