LIPOPOLYSACCHARIDES OF RALSTONIA SOLANACEARUM: FATTY ACID COMPOSITION AND BIOLOGICAL ACTIVITY

Abstract

The study of endotoxic activity indicates the relative non-toxicity (1–1.25 mg/kg) of lipopolysaccharides (LPS) from the majority of studied Ralstonia solanacearum strains comparative to enterobacterial LPS. The exception was LPS R. solanacearum 749, which showed a higher (0.4 mg/ kg) toxicity. The strains of R. solanacearum exhibited division into two groups on the basis of their LPS pyrogenic action. LPS of the first group of strains in the second hour after administration caused decreasing of the temperature in rabbits with a tendency to normalisation and during the third hour, the temperature values reached nearly baseline (R. solanacearum 749, 758, 52, 4). LPS of other strains (R. solanacearum 35, TX1 , TS3 , 7954) led to increasing of the temperature in experimental animals by more than 0.45 °C, wich overcomes the physiological norm of healthy animals. The study of R. solanacearum LPS revealed a high level of heterogenity in quantitative and qualitative fatty acid composition.

Keywords

• ліпополісахариди
• жирнокислотний склад
• ralstonia solanacearum
• токсичність
• пірогенність.