PCL/gelatin nanofibers incorporated with starfish polydeoxyribonucleotides for potential wound healing applications

Tae-Hee Kim; Se-Chang Kim; Won Sun Park; Il-Whan Choi; Hyun-Woo Kim; Hyun-Wook Kang; Young-Mog Kim; Won-Kyo Jung

Materials & Design (May 2023)

PCL/gelatin nanofibers incorporated with starfish polydeoxyribonucleotides for potential wound healing applications

Tae-Hee Kim,
Se-Chang Kim,
Won Sun Park,
Il-Whan Choi,
Hyun-Woo Kim,
Hyun-Wook Kang,
Young-Mog Kim,
Won-Kyo Jung

Affiliations

Tae-Hee Kim: Research Center for Marine-Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea
Se-Chang Kim: Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea
Won Sun Park: Department of Physiology, Kangwon National University School of Medicine, Chuncheon 24341, Republic of Korea
Il-Whan Choi: Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 47392, Republic of Korea
Hyun-Woo Kim: Research Center for Marine-Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Department of Marine Biology, Pukyong National University, Busan 48513, Republic of Korea
Hyun-Wook Kang: Research Center for Marine-Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea
Young-Mog Kim: Research Center for Marine-Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea
Won-Kyo Jung: Research Center for Marine-Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Corresponding author at: Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea.

Journal volume & issue: Vol. 229
p. 111912

Abstract

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Conventional wound dressings have several shortcomings because they are unspecific and partially effective for wound healing. To enhance the therapeutic efficiency of wound dressings, we extracted polydeoxyribonucleotides (PDRN) from Patiria pectinifera, and characterized using absorbance-based analysis, chemical composition analysis, and electrophoresis. We evaluated their cytotoxicity and wound healing activity on human dermal fibroblast (HDF) and human keratinocytes (HaCaT). Next, we used electrospinning to fabricate polycaprolactone (PCL)/gelatin (Gel)/PDRN nanofibers for wound dressing application. The average fiber diameters of PCL (P), PCL/Gel (PG), and PCL/Gel/PDRN (PGP) nanofibers were 582.88 ± 202.65, 435.65 ± 149.87, and 334.63 ± 98.09 nm, respectively. The biocompatibility of the nanofibers was assessed using MTT assay and FDA/PI staining on HDF and HaCaT, no cytotoxicity was observed. In vivo experiments with full-thickness skin defect mouse models confirmed that the PGP nanofiber accelerated the initial wound healing process, as shown by wound closure analysis and histological analysis. Our results suggest that the PGP nanofiber has potential as a biomaterial for wound dressing applications and skin tissue engineering.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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