In Vitro Targeting and Imaging of Neurogenic Differentiation in Mouse Bone-Marrow Derived Mesenchymal Stem Cells with Superparamagnetic Iron Oxide Nanoparticles

Abstract

Spinal cord injuries (SCI) are well thought to be a crucial issue that roots various side effects for a patient during their entire lifetime. Although therapeutical methods to resolve the SCI are limited, stem cell therapy is determined to be a resolving factor since it possesses the ability to induce the neurogenic differentiation and the paracrine effect. However, stem cells are difficult to inject directly into the lesion, so they must be carefully guided through the spinal canal. Therefore, superparamagnetic iron oxide nanoparticles (SPIONs) are introduced as an instigator that makes the cells respond to the applied magnetic field. This study intends to report the synthesis strategy to develop SPIONs that could be used to treat the injury site by an applied magnetic field. SPION-internalized D1 Mesenchymal stem cells (MSCs) are observed consistently using a confocal fluorescence microscope to analyze the toxicity, maintenance, and monitoring points of intracellular SPIONs. The prepared SPIONs are much anticipated to increase the migration efficiency using magnetism, which was not cytotoxic. Hence, the prepared SPIONs can adeptly target the damaged neural tissue to promote tissue regeneration and treat nervous system disorders. This primary study stands as a focal point to solve SCI by stem cell migration effectively.

Keywords

• spinal cord injuries (SCI)
• superparamagnetic iron oxide nanoparticles (SPIONs)
• migration efficiency