Directed evolution of rRNA improves translation kinetics and recombinant protein yield

Fan Liu; Siniša Bratulić; Alan Costello; Teemu P. Miettinen; Ahmed H. Badran

doi:10.1038/s41467-021-25852-5

Nature Communications (Sep 2021)

Directed evolution of rRNA improves translation kinetics and recombinant protein yield

Fan Liu,
Siniša Bratulić,
Alan Costello,
Teemu P. Miettinen,
Ahmed H. Badran

Affiliations

Fan Liu: The Broad Institute of MIT & Harvard University
Siniša Bratulić: The Broad Institute of MIT & Harvard University
Alan Costello: The Broad Institute of MIT & Harvard University
Teemu P. Miettinen: Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Ahmed H. Badran: The Broad Institute of MIT & Harvard University

DOI: https://doi.org/10.1038/s41467-021-25852-5
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Ribosome kinetics are rate-limiting for protein synthesis. Here the authors evolve diverse 16S rRNAs for enhanced protein synthesis rates and genetic code expansion efficiencies in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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