Frontiers in Microbiology (Apr 2024)

Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene

  • Qin Li,
  • Qiao Zhou,
  • Yuan Chen,
  • Kaidi Hu,
  • Montserrat Sarrà,
  • Jianlong Li,
  • Aiping Liu,
  • Likou Zou,
  • Shuliang Liu

DOI
https://doi.org/10.3389/fmicb.2024.1361335
Journal volume & issue
Vol. 15

Abstract

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As an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene sca (1,230 bp). After sca was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4′-HO-3-PBA and 3′-HO-4-PBA. In conclusion, we successfully identified and validated sca as the pivotal enzyme responsible for the efficient degradation of 3-PBA from Sphingomonas baderi, providing a crucial theoretical foundation for further explorations on the degradation potential of SC-1.

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