Química Nova (Jan 2011)

Validação de método para determinação de ácidos orgânicos voláteis em efluentes de reatores anaeróbios empregando cromatografia líquida

  • Maristela Barnes Rodrigues Cerqueira,
  • Adriana Neves Dias,
  • Sergiane Souza Caldas,
  • Fabrício Butierres Santana,
  • Marcelo Gonçalves Montes D'Oca,
  • Ednei Gilberto Primel

DOI
https://doi.org/10.1590/S0100-40422011000100029
Journal volume & issue
Vol. 34, no. 1
pp. 156 – 159

Abstract

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This work deals with the method validation for the determination of acetic, propionic and butyric acids (VFAs) in wastewaters from anaerobic reactors by HPLC-DAD. Separation was performed using a C18 column and the mobile phase composition were water pH 3.0 and methanol 90:10 (v/v). The detection and quantification was carried out at 220 nm. The method shows good linearity (r²>0.996), with adequate accuracy (89-102%) and relative standard deviations lower than 18%. The matrix effect was considered low (-4.1, -3.9 and 1.4%). The developed method is fast, simple and cheap; and it was applied in wastewater samples from anaerobic reactor.

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