Frontiers in Immunology (Aug 2023)

Comprehensive autoantibody profiling in systemic autoimmunity by a highly-sensitive multiplex protein array

  • Ai Kuzumi,
  • Yuta Norimatsu,
  • Kazuki M. Matsuda,
  • Chihiro Ono,
  • Taishi Okumura,
  • Emi Kogo,
  • Naoki Goshima,
  • Naoki Goshima,
  • Takemichi Fukasawa,
  • Takemichi Fukasawa,
  • Natsumi Fushida,
  • Motoki Horii,
  • Takashi Yamashita,
  • Asako Yoshizaki-Ogawa,
  • Kei Yamaguchi,
  • Kei Yamaguchi,
  • Takashi Matsushita,
  • Shinichi Sato,
  • Ayumi Yoshizaki,
  • Ayumi Yoshizaki

DOI
https://doi.org/10.3389/fimmu.2023.1255540
Journal volume & issue
Vol. 14

Abstract

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Comprehensive autoantibody evaluation is essential for the management of autoimmune disorders. However, conventional methods suffer from poor sensitivity, low throughput, or limited availability. Here, using a proteome-wide human cDNA library, we developed a novel multiplex protein assay (autoantibody array assay; A-Cube) covering 65 antigens of 43 autoantibodies that are associated with systemic sclerosis (SSc) and polymyositis/dermatomyositis (PM/DM). The performance of A-Cube was validated against immunoprecipitation and established enzyme-linked immunosorbent assay. Further, through an evaluation of serum samples from 357 SSc and 172 PM/DM patients, A-Cube meticulously illustrated a diverse autoantibody landscape in these diseases. The wide coverage and high sensitivity of A-Cube also allowed the overlap and correlation analysis between multiple autoantibodies. Lastly, reviewing the cases with distinct autoantibody profiles by A-Cube underscored the importance of thorough autoantibody detection. Together, these data highlighted the utility of A-Cube as well as the clinical relevance of autoantibody profiles in SSc and PM/DM.

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