Microorganisms (Jul 2022)
Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method
- Alina Tscherne,
- Enrico Mantel,
- Talar Boskani,
- Sylwia Budniak,
- Mandy Elschner,
- Antonio Fasanella,
- Siri L. Feruglio,
- Domenico Galante,
- Christian G. Giske,
- Roland Grunow,
- Judit Henczko,
- Christin Hinz,
- Wojciech Iwaniak,
- Daniela Jacob,
- Agnieszka Kedrak-Jablonska,
- Veronica K. Jensen,
- Tone B. Johansen,
- Gunnar Kahlmeter,
- Viviana Manzulli,
- Erika Matuschek,
- Falk Melzer,
- Maria S. Nuncio,
- Joseph Papaparaskevas,
- Ana Pelerito,
- Margrete Solheim,
- Susanne Thomann,
- Athanasios Tsakris,
- Tara Wahab,
- Marcin Weiner,
- Lothar Zoeller,
- Sabine Zange
Affiliations
- Alina Tscherne
- Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany
- Enrico Mantel
- Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany
- Talar Boskani
- Public Health Agency of Sweden, Nobels väg 18, 171 82 Solna, Sweden
- Sylwia Budniak
- National Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Pulawy, Poland
- Mandy Elschner
- Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany
- Antonio Fasanella
- Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, Italy
- Siri L. Feruglio
- Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway
- Domenico Galante
- Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, Italy
- Christian G. Giske
- Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Alfred Nobels Allé 8, Huddinge, 141 52 Stockholm, Sweden
- Roland Grunow
- Highly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens, Division 2 (ZBS 2), Robert Koch Institute, Seestrasse 10, 13353 Berlin, Germany
- Judit Henczko
- National Public Health Center, Albert Flórián út 2-6, 1097 Budapest, Hungary
- Christin Hinz
- Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany
- Wojciech Iwaniak
- CEDROB S.A., Ujazdowek 2A, 06-400 Ciechanow, Poland
- Daniela Jacob
- Highly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens, Division 2 (ZBS 2), Robert Koch Institute, Seestrasse 10, 13353 Berlin, Germany
- Agnieszka Kedrak-Jablonska
- National Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Pulawy, Poland
- Veronica K. Jensen
- Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway
- Tone B. Johansen
- Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway
- Gunnar Kahlmeter
- EUCAST Development Laboratory (EDL) for Bacteria, 351 85 Växjö, Sweden
- Viviana Manzulli
- Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, Italy
- Erika Matuschek
- EUCAST Development Laboratory (EDL) for Bacteria, 351 85 Växjö, Sweden
- Falk Melzer
- Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany
- Maria S. Nuncio
- National Institute of Health, Av. Padre Cruz, 1649-016 Lisbon, Portugal
- Joseph Papaparaskevas
- Microbiology Department, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, 11527 Athens, Greece
- Ana Pelerito
- National Institute of Health, Av. Padre Cruz, 1649-016 Lisbon, Portugal
- Margrete Solheim
- Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway
- Susanne Thomann
- Laboratory of Spiez, Austrasse, 3700 Spiez, Switzerland
- Athanasios Tsakris
- Microbiology Department, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, 11527 Athens, Greece
- Tara Wahab
- Public Health Agency of Sweden, Nobels väg 18, 171 82 Solna, Sweden
- Marcin Weiner
- National Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Pulawy, Poland
- Lothar Zoeller
- Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany
- Sabine Zange
- Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany
- DOI
- https://doi.org/10.3390/microorganisms10071470
- Journal volume & issue
-
Vol. 10,
no. 7
p. 1470
Abstract
Brucellosis, mainly caused by Brucella (B.) melitensis, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for B. melitensis are not available, and the agent is not yet listed in the EUCAST breakpoint tables. CLSI recommendations for B. melitensis exist, but they do not fulfill the requirements of the ISO 20776 standard regarding the culture medium and the incubation conditions. Under the third EU Health Programme, laboratories specializing in the diagnostics of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aiming to develop a suitable method for the AST of B. melitensis. Under the supervision of EUCAST representatives, this working group adapted the CLSI M45 document to the ISO 20776 standard after testing and validation. These adaptations included the comparison of various culture media, culture conditions and AST methods. A Standard Operation Procedure was derived and an interlaboratory validation was performed in order to evaluate the method. The results showed pros and cons for both of the two methods but also indicate that it is not necessary to abandon Mueller–Hinton without additives for the AST of B. melitensis.
Keywords
- <i>Brucella melitensis</i>
- antimicrobial susceptibility testing
- interlaboratory validation
- ISO 20776 standard
- CLSI
