Evaluation of miR 26b 5p changes during differentiation of human endometrial stem cells into cardiomyocytes

Abstract

Background: Recently, menstrual blood-derived stem cells as a unique source of stem cells with some features such as ease of access, high ability to proliferate and regenerate, lack of immune system stimulation and no tumorigenesis have raised great hopes for heart disease cell therapy. However, the regulatory mechanisms and role of miRNAs in controlling the differentiation of stem cells into cardiomyocytes are not fully understood. In this study, the level of human miR-26b-5p microRNA were investigated before and after differentiation of endometrial stem cells into heart cardiomyocytes. Materials and Methods: Endometrial mesenchymal stem cells were differentiated into cardiomyocyte-like cells for 30 days in the presence of 5-azacitidine and fibroblast growth factor. Then, using bioinformatics studies, human miR-26b-5p microRNA was selected and its expression pattern was performed during days 0, 6, 12, 18, 24 and 30 of differentiation by Real Time qRT PCR Results: Expression Level of human miR-26b-5p showed an uptrend differentiation between days 0 and 6 and then showed a significant decreasing trend differentiation from day 6 to day 18 and an uptrend again after day 18. Conclusion: The non-uniformity in the expression of hsa-miR-26b-5p microRNA during the 24-day differentiation induction period indicates the existence of different messaging pathways involved in the differentiation process as well as different phases in the evolution and differentiation of cardiomyocytes. The miRNAs involved in the differentiation process and their possible role in turning off and on these messaging pathways at the beginning or end of a phase seems necessary.

Keywords

• endometrial mesenchymal stem cells
• cardiomyocyte
• human mirn26a microrna
• cell differentiation