Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells

Hongbao Fang; Shanshan Geng; Mingang Hao; Qixin Chen; Minglun Liu; Chunyan Liu; Zhiqi Tian; Chengjun Wang; Takanori Takebe; Jun-Lin Guan; Yuncong Chen; Zijian Guo; Weijiang He; Jiajie Diao

doi:10.1038/s41467-020-20309-7

Nature Communications (Jan 2021)

Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells

Hongbao Fang,
Shanshan Geng,
Mingang Hao,
Qixin Chen,
Minglun Liu,
Chunyan Liu,
Zhiqi Tian,
Chengjun Wang,
Takanori Takebe,
Jun-Lin Guan,
Yuncong Chen,
Zijian Guo,
Weijiang He,
Jiajie Diao

Affiliations

Hongbao Fang: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University
Shanshan Geng: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University
Mingang Hao: Department of Cancer Biology, University of Cincinnati College of Medicine
Qixin Chen: Department of Cancer Biology, University of Cincinnati College of Medicine
Minglun Liu: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University
Chunyan Liu: Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children’s Hospital Medical Center
Zhiqi Tian: Department of Cancer Biology, University of Cincinnati College of Medicine
Chengjun Wang: Sinopec Shengli Petroleum Engineering Limited Company
Takanori Takebe: Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children’s Hospital Medical Center
Jun-Lin Guan: Department of Cancer Biology, University of Cincinnati College of Medicine
Yuncong Chen: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University
Zijian Guo: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University
Weijiang He: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University
Jiajie Diao: Department of Cancer Biology, University of Cincinnati College of Medicine

DOI: https://doi.org/10.1038/s41467-020-20309-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Subcellular Zn2+ compartmentalisation is essential for cell biology. Here the authors make a turn-on fluorescent Zn2+ probe that localises to multiple organelles, and correlate its location using organelle morphology derived from structured illumination microscopy.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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