qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

Richard M. Giadone; Aditya Mithal; Gregory J. Miller; Taylor M. Matte; Anthony K. Yeung; Todd W. Dowrey; Rhiannon B. Werder; Nancy S. Miller; Christopher D. Andry; Kim Vanuytsel; George J. Murphy

STAR Protocols (Sep 2020)

qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

Richard M. Giadone,
Aditya Mithal,
Gregory J. Miller,
Taylor M. Matte,
Anthony K. Yeung,
Todd W. Dowrey,
Rhiannon B. Werder,
Nancy S. Miller,
Christopher D. Andry,
Kim Vanuytsel,
George J. Murphy

Affiliations

Richard M. Giadone: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; Corresponding author
Aditya Mithal: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA
Gregory J. Miller: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA
Taylor M. Matte: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA
Anthony K. Yeung: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA
Todd W. Dowrey: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA
Rhiannon B. Werder: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA
Nancy S. Miller: Department of Pathology and Laboratory Medicine, Boston Medical Center and Boston University School of Medicine, Boston, MA 02218, USA
Christopher D. Andry: Department of Pathology and Laboratory Medicine, Boston Medical Center and Boston University School of Medicine, Boston, MA 02218, USA
Kim Vanuytsel: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; Section of Hematology and Oncology, Department of Medicine, Boston University School of Medicine, Boston, MA 02218, USA; Corresponding author
George J. Murphy: Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; Section of Hematology and Oncology, Department of Medicine, Boston University School of Medicine, Boston, MA 02218, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 2
p. 100102

Abstract

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Summary: The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time.For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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