Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke

Damjan Osredkar; Jeffrey W. Sall; Philip E. Bickler; Donna M. Ferriero

Neurobiology of Disease (May 2010)

Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke

Damjan Osredkar,
Jeffrey W. Sall,
Philip E. Bickler,
Donna M. Ferriero

Affiliations

Damjan Osredkar: Department of Neurology, University of California Box 0663, 521 Parnassus Avenue C215, San Francisco, CA 94143-0663, USA; Department of Pediatric Neurology, University Children's Hospital, Ljubljana, Slovenia
Jeffrey W. Sall: Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA
Philip E. Bickler: Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA
Donna M. Ferriero: Department of Neurology, University of California Box 0663, 521 Parnassus Avenue C215, San Francisco, CA 94143-0663, USA; Department of Pediatrics, University of California, San Francisco, CA, USA; Corresponding author. Department of Neurology, University of California Box 0663, 521 Parnassus Avenue C215, San Francisco, CA 94143-0663, USA. Fax: +1 415 502 5821.

Journal volume & issue: Vol. 38, no. 2
pp. 259 – 265

Abstract

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The hippocampus is often injured in neonatal stroke. We have investigated the effect of erythropoietin (EPO) on oxygen-glucose deprived hippocampal slices and hypoxic progenitor cells. EPO improved survival of the organotypic hippocampal slices with significantly less cell death in the dentate gyrus and an increased number of proliferating cells 4–5 days after insult. Significantly fewer markers of neurogenesis were seen after the insult but when EPO was added to the culture medium, neurogenesis was sustained. When hippocampal progenitor cultures were stimulated into differentiation, more cells chose a neuronal cell fate when treated with EPO. These findings support the hypothesis that EPO not only prevents ischemia induced cell death but promotes neuronal cell fate commitment in in vitro models of neonatal stroke.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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