Transcriptional silencing of long noncoding RNA GNG12-AS1 uncouples its transcriptional and product-related functions

Lovorka Stojic; Malwina Niemczyk; Arturo Orjalo; Yoko Ito; Anna Elisabeth Maria Ruijter; Santiago Uribe-Lewis; Nimesh Joseph; Stephen Weston; Suraj Menon; Duncan T. Odom; John Rinn; Fanni Gergely; Adele Murrell

doi:10.1038/ncomms10406

Nature Communications (Feb 2016)

Transcriptional silencing of long noncoding RNA GNG12-AS1 uncouples its transcriptional and product-related functions

Lovorka Stojic,
Malwina Niemczyk,
Arturo Orjalo,
Yoko Ito,
Anna Elisabeth Maria Ruijter,
Santiago Uribe-Lewis,
Nimesh Joseph,
Stephen Weston,
Suraj Menon,
Duncan T. Odom,
John Rinn,
Fanni Gergely,
Adele Murrell

Affiliations

Lovorka Stojic: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Malwina Niemczyk: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Arturo Orjalo: Biosearch Technologies Inc.
Yoko Ito: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Anna Elisabeth Maria Ruijter: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Santiago Uribe-Lewis: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Nimesh Joseph: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Stephen Weston: Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Claverton Down
Suraj Menon: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Duncan T. Odom: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
John Rinn: Department of Stem Cell and Regenerative Biology, Harvard University
Fanni Gergely: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way
Adele Murrell: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way

DOI: https://doi.org/10.1038/ncomms10406
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 14

Abstract

Read online

LncRNAs regulate gene expression via their RNA product and through transcriptional interference. Here, Stojic et al.uncouple these functions by using multiple siRNAs against GNG12-AS1 to show that this lncRNA has a product related role in MET signaling while its transcription modulates DIRAS3 expression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal