Normal and reversed phase high performance liquid chromatography of some new 1, 2, 4-triazole derivatives

Abstract

The retention behaviour and separation ability of normal and reversed phase HPLC with one non-polar and two polar mobile phases, have been studied by measuring the retention constants of a series of newly synthesized 1,2,4-triazole derivatives. The results are discussed in terms of the nature of the solute, eluent and stationary phase.

Keywords

• High performance liquid chromatography (HPLC)
• normal phase
• silica gel column
• reversed phase
• C-8 bonded silica gel column
• 1,2,4-triazole derivatives