Nature Communications (Aug 2020)

High throughput error corrected Nanopore single cell transcriptome sequencing

  • Kevin Lebrigand,
  • Virginie Magnone,
  • Pascal Barbry,
  • Rainer Waldmann

DOI
https://doi.org/10.1038/s41467-020-17800-6
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 8

Abstract

Read online

Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full length sequences.