Purification of Colon Carcinoma Cells from Primary Colon Tumor Using a Filtration Method via Porous Polymeric Filters

Jia-Hua Wang; Lee-Kiat Ban; Henry Hsin-Chung Lee; Yen-Hung Chen; Hui-Yu Lin; Zhe-Wei Zhu; Her-Young Su; Akihiro Umezawa; Abdulrahman I. Almansour; Natarajan Arumugam; Raju Suresh Kumar; Gwo-Jang Wu; Akon Higuchi

doi:10.3390/polym13193411

Polymers (Oct 2021)

Purification of Colon Carcinoma Cells from Primary Colon Tumor Using a Filtration Method via Porous Polymeric Filters

Jia-Hua Wang,
Lee-Kiat Ban,
Henry Hsin-Chung Lee,
Yen-Hung Chen,
Hui-Yu Lin,
Zhe-Wei Zhu,
Her-Young Su,
Akihiro Umezawa,
Abdulrahman I. Almansour,
Natarajan Arumugam,
Raju Suresh Kumar,
Gwo-Jang Wu,
Akon Higuchi

Affiliations

Jia-Hua Wang: Department of Chemical and Materials Engineering, National Central University, Taoyuan 320, Taiwan
Lee-Kiat Ban: Department of Surgery, Hsinchu Cathay General Hospital, Hsinchu 300, Taiwan
Henry Hsin-Chung Lee: Department of Surgery, Hsinchu Cathay General Hospital, Hsinchu 300, Taiwan
Yen-Hung Chen: Department of Chemical and Materials Engineering, National Central University, Taoyuan 320, Taiwan
Hui-Yu Lin: Department of Chemical and Materials Engineering, National Central University, Taoyuan 320, Taiwan
Zhe-Wei Zhu: Department of Chemical and Materials Engineering, National Central University, Taoyuan 320, Taiwan
Her-Young Su: Department of Obstetrics and Gynecology, Bobson Yuho Women and Children’s Clinic, Hsinchu 302, Taiwan
Akihiro Umezawa: Department of Reproduction, National Center for Child Health and Development, Tokyo 157-8535, Japan
Abdulrahman I. Almansour: Department of Chemistry, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia
Natarajan Arumugam: Department of Chemistry, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia
Raju Suresh Kumar: Department of Chemistry, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia
Gwo-Jang Wu: Graduate Institute of Medical Sciences and Department of Obstetrics & Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan
Akon Higuchi: Department of Chemical and Materials Engineering, National Central University, Taoyuan 320, Taiwan

DOI: https://doi.org/10.3390/polym13193411
Journal volume & issue: Vol. 13, no. 19
p. 3411

Abstract

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Cancer stem cells (CSCs) or cancer-initiating cells (CICs) are key factors for tumor generation and metastasis. We investigated a filtration method to enhance CSCs (CICs) from colon carcinoma HT-29 cells and primary colon carcinoma cells derived from patient colon tumors using poly(lactide-co-glycolic acid)/silk screen (PLGA/SK) filters. The colon carcinoma cell solutions were permeated via porous filters to obtain a permeation solution. Then, the cell cultivation media were permeated via the filters to obtain the recovered solution, where the colon carcinoma cells that adhered to the filters were washed off into the recovered solution. Subsequently, the filters were incubated in the culture media to obtain the migrated cells via the filters. Colon carcinoma HT-29 cells with high tumorigenicity, which might be CSCs (CICs), were enhanced in the cells in the recovered solution and in the migrated cells based on the CSC (CIC) marker expression, colony-forming unit assay, and carcinoembryonic antigen (CEA) production. Although primary colon carcinoma cells isolated from colon tumor tissues contained fibroblast-like cells, the primary colon carcinoma cells were purified from fibroblast-like cells by filtration through PLGA/SK filters, indicating that the filtration method is effective in purifying primary colon carcinoma cells.

Published in Polymers

ISSN: 2073-4360 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/polymers/

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