International Journal of Biomaterials (Jan 2012)

Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility

  • Martina Schleicher,
  • Jan Hansmann,
  • Bentsian Elkin,
  • Petra J. Kluger,
  • Simone Liebscher,
  • Agnes J. T. Huber,
  • Olaf Fritze,
  • Christine Schille,
  • Michaela Müller,
  • Katja Schenke-Layland,
  • Martina Seifert,
  • Heike Walles,
  • Hans-Peter Wendel,
  • Ulrich A. Stock

DOI
https://doi.org/10.1155/2012/397813
Journal volume & issue
Vol. 2012

Abstract

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In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m2) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P<0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.