Cloning of MYB10, PAL and UFGT genes from ‘Cuihongli’ and ‘Qiangcuili’

Abstract

PAL, UFGT as structural genes and MYB10 as a regulatory gene play an important role in the accumulation of anthocyanins in plants. In this experiment, ‘Cuihongli’ and ‘Qiangcuili’ were used as materials to clone PAL, UFGT and MYB10 related to anthocyanin synthesis by homologous sequence cloning. The results showed that the full length of PAL was 2160 bp, encoding 719 amino acids; the full length of UFGT was 1428 bp, encoding 475 amino acids, and the differences of PAL and UFGT between the two cultivars were one amino acid and three amino acids, respectively. The sequence length of ‘Cuihongli’ MYB10 gene is 732 bp, which encodes 243 amino acids and belongs to the superfamily of SANT family. Homology analysis and phylogenetic tree analysis showed that the proteins encoded by PAL, UFGT and MYB10 genes were short and closely related to Prunus persica, Prunus avium, Prunus armeniaca and Prunus cerasifera in Rosaceae.