Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats

G. Ozbey; A. Kilic; H. B Ertas; A. Muz

doi:10.17221/5678-VETMED

Veterinární Medicína (Mar 2004)

Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats

G. Ozbey,
A. Kilic,
H. B Ertas,
A. Muz

Affiliations

G. Ozbey: Department of Microbiology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey
A. Kilic: Department of Microbiology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey
H. B Ertas: Department of Microbiology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey
A. Muz: Department of Microbiology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey

DOI: https://doi.org/10.17221/5678-VETMED
Journal volume & issue: Vol. 49, no. 3
pp. 65 – 69

Abstract

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n this study, 30, 15 and 1 strains of Pasteurella multocida and 9, 8 and 6 strains of Mannheimia haemolytica from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of P. multocida isolates from cattle and sheep, respectively. However, M. haemolytica isolates from cattle, sheep and goats showed only one profile and these strains were not discriminated by RAPD. This study showed that little genetic heterogeneity exists among P. multocida and M. haemolytica isolates from lungs of cattle, sheep and goats.

Published in Veterinární Medicína

ISSN: 0375-8427 (Print); 1805-9392 (Online)
Publisher: Czech Academy of Agricultural Sciences
Country of publisher: Czechia
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://vetmed.agriculturejournals.cz/

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