New data on trophic associations of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) and lemurs (Primates, Lemuroidea) in Madagascar revealed by metabarcoding

Abstract

Resource use and diet specialisation of Madagascan dung beetles have been little studied especially concerning the possible associations between specific dung beetle and lemur species. Pilot studies have demonstrated that amplicon sequencing is a promising tool for the lemur inventories. In the present contribution, we report the results of the gut content analysis of three endemic Madagascan dung beetles species: Helictopleurus clouei (Harold), Epilissus apotolamproides (Lebis) and Nanos dubitatus (Lebis). Amplicon metagenomics revealed trophic associations of these species with Eulemur sanfordi (Archbold), Eu. fulvus (É.Geoffroy Saint-Hilaire) and Cheirogaleus crossleyi (Grandidier), respectively. The reads of other mammal species, revealed by the analysis, including putative contaminations, are discussed.

Keywords

• amplicon sequencing
• next-generation sequencing
• m