The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure

Izabela Koniuszewska; Monika Harnisz; Ewa Korzeniewska; Małgorzata Czatzkowska; Jan Paweł Jastrzębski; Łukasz Paukszto; Sylwia Bajkacz; Ewa Felis; Paulina Rusanowska

doi:10.3390/en14041125

Energies (Feb 2021)

The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure

Izabela Koniuszewska,
Monika Harnisz,
Ewa Korzeniewska,
Małgorzata Czatzkowska,
Jan Paweł Jastrzębski,
Łukasz Paukszto,
Sylwia Bajkacz,
Ewa Felis,
Paulina Rusanowska

Affiliations

Izabela Koniuszewska: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland
Monika Harnisz: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland
Ewa Korzeniewska: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland
Małgorzata Czatzkowska: Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland
Jan Paweł Jastrzębski: Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland
Łukasz Paukszto: Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland
Sylwia Bajkacz: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland
Ewa Felis: Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland
Paulina Rusanowska: Department of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Warszawska 117 Str., 10-950 Olsztyn, Poland

DOI: https://doi.org/10.3390/en14041125
Journal volume & issue: Vol. 14, no. 4
p. 1125

Abstract

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This study explored the effect of eight antimicrobials on the efficiency of biogas production in the anaerobic digestion (AD) process of cattle manure. The microbiome involved in AD, presence and number of genes mcrA, MSC and MST specific for Archaea, and antibiotic resistance genes (ARGs) concentration in digestate (D) were examined. Supplementation of antibiotics to substrate significantly lowered biogas production. Amoxicillin caused a 75% decrease in CH4 production in comparison with the control samples. Enrofloxacin, tetracycline, oxytetracycline, and chlortetracycline reduced the amount of biogas produced by 36, 39, 45 and 53%, respectively. High-throughput sequencing of 16S rRNA results revealed that bacteria dominated the Archaea microorganisms in all samples. Moreover, antibiotics led to a decrease in the abundance of the genes mcrA, MSC, MST, and induced an increase in the number of tetracyclines resistance genes. Antibiotics decreased the efficiency of the AD process and lowered the quantity of CH4 obtained, while stimulating an increase in the number of ARGs in D. This work reveals how antimicrobials affect the cattle manure AD process and changes in microbial biodiversity, number of functional genes and ARGs in the digestate due to drugs exposure. It also, provides useful, practical information about the AD process.

Published in Energies

ISSN: 1996-1073 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology
Website: http://www.mdpi.com/journal/energies

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