Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel

Dona Imanga Upamadi Edirisinghe; Areetha D’Souza; Maryam Ramezani; Robert J. Carroll; Quenten Chicón; Cheyene L. Muenzel; Jonathan Soule; Mary Beth Browning Monroe; Alison E. Patteson; Olga V. Makhlynets

doi:10.3390/molecules28114390

Molecules (May 2023)

Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel

Dona Imanga Upamadi Edirisinghe,
Areetha D’Souza,
Maryam Ramezani,
Robert J. Carroll,
Quenten Chicón,
Cheyene L. Muenzel,
Jonathan Soule,
Mary Beth Browning Monroe,
Alison E. Patteson,
Olga V. Makhlynets

Affiliations

Dona Imanga Upamadi Edirisinghe: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA
Areetha D’Souza: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA
Maryam Ramezani: Biomedical and Chemical Engineering, Syracuse University, Bowne Hall, Syracuse, NY 13210, USA
Robert J. Carroll: Department of Physics, Syracuse University, Syracuse, NY 13210, USA
Quenten Chicón: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA
Cheyene L. Muenzel: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA
Jonathan Soule: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA
Mary Beth Browning Monroe: Biomedical and Chemical Engineering, Syracuse University, Bowne Hall, Syracuse, NY 13210, USA
Alison E. Patteson: Department of Physics, Syracuse University, Syracuse, NY 13210, USA
Olga V. Makhlynets: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA

DOI: https://doi.org/10.3390/molecules28114390
Journal volume & issue: Vol. 28, no. 11
p. 4390

Abstract

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A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G′ was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed can have a practical application and be a paradigm shift in the approach to wound treatment, and it will improve healing outcomes for millions of patients each year.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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