Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells

Yeon Kim; Hyun-Joo Park; Mi-Kyoung Kim; Yong-Il Kim; Hyung Joon Kim; Soo-Kyung Bae; Jacques E. Nör; Moon-Kyoung Bae

Journal of Dental Sciences (Apr 2023)

Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells

Yeon Kim,
Hyun-Joo Park,
Mi-Kyoung Kim,
Yong-Il Kim,
Hyung Joon Kim,
Soo-Kyung Bae,
Jacques E. Nör,
Moon-Kyoung Bae

Affiliations

Yeon Kim: Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea
Hyun-Joo Park: Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea
Mi-Kyoung Kim: Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea
Yong-Il Kim: Department of Orthodontics, School of Dentistry, Pusan National University, Yangsan, South Korea
Hyung Joon Kim: Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea
Soo-Kyung Bae: Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea; Department of Dental Pharmacology, School of Dentistry, Pusan National University, Yangsan, South Korea
Jacques E. Nör: Department of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA
Moon-Kyoung Bae: Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea; Corresponding author. Department of Oral Physiology, School of Dentistry, Pusan National University, 49 Busandaehak-ro, Yangsan 50612, South Korea.

Journal volume & issue: Vol. 18, no. 2
pp. 577 – 585

Abstract

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Background/purpose: Naringenin, a naturally occurring flavanone in citrus fruits, regulates bone formation by bone marrow-derived mesenchymal stem cells. The purpose of this study was to characterize the effects of naringenin on some biological behaviors of human dental pulp stem cells (HDPSCs). Materials and methods: HDPSCs were cultured in osteogenic differentiation medium and osteo/odontogenic differentiation and mineralization were analyzed by alkaline phosphatase (ALP) staining and Alizarin Red S (ARS) staining. The migration of HDPSCs was evaluated by transwell chemotactic migration assays and scratch wound healing migration assay. Using tooth slice/scaffold model, we assessed the in vivo odontogenic differentiation potential of HDPSCs. Results: We have demonstrated that naringenin increases the osteogenic/odontogenic differentiation of HDPSCs through regulation of osteogenic-related proteins and the migratory ability of HDPSCs through stromal cell derived factor-1 (SDF-1)/C-X-C chemokine receptor type 4 (CXCR4) axis. Moreover, naringenin promotes the expression of dentin matrix acidic phosphoprotein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) in HDPSCs seeded on tooth slice/scaffolds that are subcutaneously implanted into immunodeficient mice. Conclusion: Our present study suggests that naringenin promotes migration and osteogenic/odontogenic differentiation of HDPSCs and may serve as a promising candidate in dental tissue engineering and bone regeneration.

Published in Journal of Dental Sciences

ISSN: 1991-7902 (Print)
Publisher: Elsevier
Country of publisher: Taiwan, Province of China
LCC subjects: Medicine: Dentistry
Website: http://www.journals.elsevier.com/journal-of-dental-sciences/

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