Heliyon (Mar 2024)

Chemical composition and antimicrobial study of Crossobamon orientalis body oil

  • Shakeel Ahmad,
  • Kashif Ali,
  • Khalil Ahmad,
  • Tahira Ruby,
  • Hammad Majeed,
  • Muhammad Qamar Saeed,
  • Mudassar Fareed Awan,
  • Irfan Ahmad,
  • Muhammad Farooq,
  • Mirza Imran Shahzad,
  • Saad Alamri,
  • Aleem Ahmed Khan

Journal volume & issue
Vol. 10, no. 6
p. e28225

Abstract

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Geckos and their products have been used in Asian traditional medicine. Medicinal properties of desert-dwelling Gecko species, Crossobamon orientalis remain unexplored. In this study, natural bioactive macromolecules present in oil extracted from C. orientalis (COO) and their biological activities were evaluated. Chemical constitution of COO was explored by using gas chromatography mass spectrometry. Antioxidant, antiviral, and antibacterial activities of COO extracts were assessed using various assays, including DPPH free-radical-protocol, HET-CAM method, in ovo-antiviral technique, and disc-diffusion method. GC-MS study reported 40 different compounds in COO. n-hexane and methanol extracts of COO demonstrated highest DPPH radical inhibition, with values of 70 and 63.3%, respectively. Extracts of COO in solvents, namely 1-butanol, methanol, diethyl ether, and n-hexane significantly inhibited the proliferation of four pathogenic viruses. Maximum zone of inhibition was observed for Escherichia coli (13.65 ± 0.57 mm). These findings suggest that COO possesses potent antioxidant and antimicrobial properties against viral and bacterial strains, thanks to its biologically active components having no side effects. Further studies are essential to isolate and identify individual bioactive compounds present in COO and to investigate their potential as therapeutic agents.

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