Protocol for differentiation of monocytes and macrophages from human induced pluripotent stem cells

Kevin Emmerich; Francesca Calcaterra; Xuming Tang; Guibin Chen; Elena Pontarini; Roberta Ciceri; Dan Yang; Benjamin Joseph; Silvia Della Bella; Isabella Varea; Domenico Mavilio; Manfred Boehm

STAR Protocols (Sep 2024)

Protocol for differentiation of monocytes and macrophages from human induced pluripotent stem cells

Kevin Emmerich,
Francesca Calcaterra,
Xuming Tang,
Guibin Chen,
Elena Pontarini,
Roberta Ciceri,
Dan Yang,
Benjamin Joseph,
Silvia Della Bella,
Isabella Varea,
Domenico Mavilio,
Manfred Boehm

Affiliations

Kevin Emmerich: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA; Corresponding author
Francesca Calcaterra: Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine (BioMeTra), University of Milan, 20054 Segrate, Italy
Xuming Tang: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA
Guibin Chen: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA
Elena Pontarini: Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy; Center for Experimental Medicine and Rheumatology, William Harvey Research Institute, London, UK
Roberta Ciceri: Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine (BioMeTra), University of Milan, 20054 Segrate, Italy
Dan Yang: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA
Benjamin Joseph: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA
Silvia Della Bella: Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine (BioMeTra), University of Milan, 20054 Segrate, Italy
Isabella Varea: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA
Domenico Mavilio: Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine (BioMeTra), University of Milan, 20054 Segrate, Italy
Manfred Boehm: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 3
p. 103217

Abstract

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Summary: Study of disease-relevant immune cells, namely monocytes and macrophages, is limited based on availability of primary tissue, a limitation that can be remedied using human induced pluripotent stem cell (hiPSC) technology. Here, we present a protocol for differentiation of monocytes and macrophages from hiPSCs. We describe steps for hiPSC maintenance, mesoderm lineage induction, hematopoietic progenitor cells (HPCs) commitment and expansion, and myeloid lineage induction. We then detail procedures for monocyte formation and functional macrophage formation and polarization.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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