康复学报 (Apr 2019)
Expression and Clinical Significance of DAP3 in Breast Cancer and Effect of Bruceine D on Expression of DAP3:Analysis Based on Data-mining from Bioinformatics
Abstract
Objective:To clarify the expression and significance of DAP3 in breast cancer, and further explore the effect of Bruceine D on the expression of DAP3 gene in breast cancer cells.Methods:The datasets about the expression of DAP3 gene in breast cancer were retrieved and mined from Oncomine and GEO databases, and the relationship of these datasets and the prognosis value of DAP3 in breast cancer was retrieved from an online survival analysis tool"Kaplan-Meier Plotter"database in order to explore the relationship between these datasets and the prognosis of breast cancer. The expression of DAP3 in breast cancer cell lines was analyzed by using CCLE database to further analyze the effect of Bruceine D on the expression level of DAP3 gene in breast cancer cells.Results:In Oncomine database, there were 417 data sets of different types of DAP3 gene expression, of which 22 studies were of statistical differences(22 up-regulated). A total of nine studies were involved the expression of DAP3 in breast cancer tissues and normal tissues, including a total of 1 118 samples, including the comparison of invasive ductal breast carcinoma, invasive lobular breast carcinoma, invasive ductal and invasive lobular mixed breast carcinoma, tubular breast cancer and ductal breast carcinoma and the normal tissue. On-line analysis of nine DAP3 gene datasets collected from Oncomine database showed that the expression of DAP3 gene in breast cancer tissues was distinctly higher than that of breast adjacent normal tissues(P<0.05).Moreover, Kaplan-Meier plotter of overall survival indicated that the breast cancer patients with high expression of DAP3 have a worse survival, and the breast cancer patients with low expression of DAP3 have a better survival(P<0.05).Further subgroup analysis revealed that the expression of DAP3 had a significant effect(P<0.05)on the prognosis of breast cancer patients except for basal-like subtype, estrogen receptor-negative subtype and human epidermal growth factor receptor-2 overexpression subtype breast cancer patients. In addition, CCLE database analysis indicated that DAP3 was also highly expressed in breast cancer cell lines and its mRNA expression ranked ahead in all kinds of cancer cell lines. Finally, Bruceine D can down-regulate the DAP3 gene expression in breast cancer cells, which was found by analyzing the datasets of GSE85871 in the GEO database(P<0.05).Conclusion:DAP3 gene is high-expressed in breast cancer and its expressionlevel is closely associated with the prognosis of breast cancer patients, which may be a new intervening target of Bruceine D on breast cancer cells.
