Nature Communications (Feb 2021)
The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
- Fatemeh Askarian,
- Satoshi Uchiyama,
- Helen Masson,
- Henrik Vinther Sørensen,
- Ole Golten,
- Anne Cathrine Bunæs,
- Sophanit Mekasha,
- Åsmund Kjendseth Røhr,
- Eirik Kommedal,
- Judith Anita Ludviksen,
- Magnus Ø. Arntzen,
- Benjamin Schmidt,
- Raymond H. Zurich,
- Nina M. van Sorge,
- Vincent G. H. Eijsink,
- Ute Krengel,
- Tom Eirik Mollnes,
- Nathan E. Lewis,
- Victor Nizet,
- Gustav Vaaje-Kolstad
Affiliations
- Fatemeh Askarian
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Satoshi Uchiyama
- Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, UC San Diego
- Helen Masson
- Department of Pediatrics, University of California, San Diego, School of Medicine
- Henrik Vinther Sørensen
- Department of Chemistry, University of Oslo
- Ole Golten
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Anne Cathrine Bunæs
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Sophanit Mekasha
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Åsmund Kjendseth Røhr
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Eirik Kommedal
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Judith Anita Ludviksen
- Research Laboratory, Nordland Hospital
- Magnus Ø. Arntzen
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Benjamin Schmidt
- Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, UC San Diego
- Raymond H. Zurich
- Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, UC San Diego
- Nina M. van Sorge
- Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University
- Vincent G. H. Eijsink
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- Ute Krengel
- Department of Chemistry, University of Oslo
- Tom Eirik Mollnes
- Research Laboratory, Nordland Hospital
- Nathan E. Lewis
- Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, UC San Diego
- Victor Nizet
- Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, UC San Diego
- Gustav Vaaje-Kolstad
- Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU)
- DOI
- https://doi.org/10.1038/s41467-021-21473-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 19
Abstract
The Pseudomonas aeruginosa lytic polysaccharide monooxygenase CbpD, prevalent in clinical isolates, has been proposed to act as a virulence factor. Here, the authors combine structural work, in silico simulations, enzymatic activity and in vitro and in vivo experiments to further delineate the role of CbpD and show that its deletion renders P. aeruginosa unable to establish a lethal systemic infection, leading to enhanced bacterial clearance in a mouse model of infection.
