Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong

Yuan-Feng Zou; Yu-Ping Fu; Xing-Fu Chen; Ingvild Austarheim; Kari Tvete Inngjerdingen; Chao Huang; Lemlem Dugassa Eticha; Xu Song; Lixia Li; Bin Feng; Chang-Liang He; Zhong-Qiong Yin; Berit Smestad Paulsen

doi:10.3390/molecules22020287

Molecules (Feb 2017)

Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong

Yuan-Feng Zou,
Yu-Ping Fu,
Xing-Fu Chen,
Ingvild Austarheim,
Kari Tvete Inngjerdingen,
Chao Huang,
Lemlem Dugassa Eticha,
Xu Song,
Lixia Li,
Bin Feng,
Chang-Liang He,
Zhong-Qiong Yin,
Berit Smestad Paulsen

Affiliations

Yuan-Feng Zou: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Yu-Ping Fu: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Xing-Fu Chen: Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China
Ingvild Austarheim: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway
Kari Tvete Inngjerdingen: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway
Chao Huang: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Lemlem Dugassa Eticha: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway
Xu Song: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Lixia Li: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Bin Feng: Animal Nutrition Institute, Sichuan Agricultural University, Wenjiang 611130, China
Chang-Liang He: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Zhong-Qiong Yin: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
Berit Smestad Paulsen: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway

DOI: https://doi.org/10.3390/molecules22020287
Journal volume & issue: Vol. 22, no. 2
p. 287

Abstract

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Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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