Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in <i>Saccharomyces cerevisiae</i>

Satoshi Okada; Goro Doi; Shitomi Nakagawa; Emiko Kusumoto; Takashi Ito

doi:10.1093/g3journal/jkab304

G3: Genes, Genomes, Genetics (Aug 2021)

Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in <i>Saccharomyces cerevisiae</i>

Satoshi Okada,
Goro Doi,
Shitomi Nakagawa,
Emiko Kusumoto,
Takashi Ito

Affiliations

Satoshi Okada: ORCiD; Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan
Goro Doi: Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan
Shitomi Nakagawa: Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan
Emiko Kusumoto: Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan
Takashi Ito: ORCiD; Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan

DOI: https://doi.org/10.1093/g3journal/jkab304
Journal volume & issue: Vol. 11, no. 12

Abstract

Read online

AbstractGenome editing using the CRISPR/Cas system has been implemented for various organisms and becomes increasingly popular even in the genetically tractable budding yeast Saccharomyces cerevisiae

Published in G3: Genes, Genomes, Genetics

ISSN: 2160-1836 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://academic.oup.com/g3journal

About the journal